ELISpot Path: CMV Human IFN-γ

ELISpot Path: CMV Human IFN-γ

3420-2AST-P2-1

Publications: 2
Documents
Questions?
A straightforward setup for functional analysis of a specific scientific question.

Components

Detection mAb7-B6-1, ALP
SubstrateBCIP/NBT-plus
PlatePre-coated ELISpot plate, strip (mAb 1-D1K)
Positive controlAnti-CD3 mAb (CD3-2)
Peptide poolCMV (CD4 and CD8), human

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Path kit is intended for the enumeration of cells secreting human IFN-γ in response to CMV peptides using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Peptide pool included in the kit
The CMV peptide pool contains 42 peptides derived from CMV. The antigens represented in the peptide pool are pp50, pp65, IE1, IE2, and Envelope glycoprotein B. Of the included peptides, 28 are MHC class I restricted and 14 are MHC class II restricted.

Recommendation

Product details

ProductELISpot Path: CMV Human IFN-γ
ApplicationELISpot
AnalyteIFN-γ
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IFN-γ.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C. Peptide pools should be stored at -20 °C or below. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.

Human PBMC (250,000 cells/well) were incubated overnight in the absence or presence of the CMV peptide pool for human CD4 and CD8 T cells (Mabtech) and the number of cells secreting IFN-γ was analyzed by ELISpot.

Datasheet/Protocol
SDS

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them
ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs
This is a protocol on how to best isolate, freeze, and thaw PBMCs.
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell typeT cell, Tc, Th1, NK cell
Gene ID3458
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FluoroSpot Path: CMV Human IFN-γ/IL-2